5.3 Using formulae to calculate chlorophyll |
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Creating a bit-mask Calculating FLH Calculating chlorophyll concentrations |
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Downloads: Images: MER_RR_2COLRA 200402~.N1 Description
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In this section you will investigate the relationship between the algal_1 pigment index (chlorophyll) and fluorescence line height (FLH). FLH is an indicator of solar induced chlorophyll fluorescence, an is expected to increase as chlorophyll concentration increases. However, the relationship is not necessarily straight forward for a number of reasons:
Never-the-less, FLH may be a useful indicator of chlorophyll, and the MERIS channels 7, 8 and 9 were chosen so as to provide a measure of the chlorophyll fluorescence peak. We may therefore expect FLH and chlorophyll concentration to be correlated in some way. Before you carry out the calculations, however, it makes sense to create a bit-mask, which may be used to mask the output of formulae used to calculate FLH and chlorophyll, and may also be used to mask multiple bands in one go. Creating a bit-mask
This type of image, consisting of either 0 or 1, could be saved in one bit, and is therefore known as a bit-mask. A bit-mask may easily be used to remove invalid data from any type of image, regardless of numerical format, simply by multiplying the image plane containing the original data by the image plane containing the mask. We can take advantage of this by calculating FLH and masking the result of the calculations in one go.
Calculating FLHFor MERIS fluorescence line height (FLH) is defined by the following relationship:
where λ1, λ3 is the fluorescence spectral range, i.e. the bands immediately outside the fluorescence peak, MERIS bands 7 and 9; λ2 is the fluorescence emission band (band 8); L2, L3 is the radiance of the baseline, and L2 is the radiance in the fluorescence band. By using reflectances instead of radiances, we will to some extent compensate for the relationship between fluorescence and incident light; thus making it likely that the calculated FLH will correlate more strongly with chlorophyll.
Calculating chlorophyll concentrationsThe algal_1 data has been saved as 8-bit integer data. The relationship between these numerical values and the chlorophyll concentrations may be found in the 'properties' of this data set, which you can open by right-clicking on the algal_1 folder in right frame of the N1 file structure window. (Note that you cannot have the algal_1 data opened as an image when you do this.) |
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